Vitrolife EmbryoScope - Calibrating the Temperature

EmbryoScope time-lapse incubator
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The core temperature of the culture dish holder measured by the temperature probe is
0.2°C higher than the temperature at the bottom of the microwell where the embryos reside
as specified in the following table:
Core temperature of the culture dish
holder measured by the external probe
(°C)
Temperature at the bottom of the
microwell (medium temperature, °C)
37.00
36.80
37.10
36.90
37.20
37.00
37.30
37.10
37.40
37.20
37.50
37.30
5.4.3 Calibrating the temperature
The EmbryoScope time-lapse incubator must have been turned on for at least three hours to
achieve full equilibrium before the temperature is calibrated. The room temperature must be
equivalent to normal laboratory temperature.
If the external temperature measured by using the high-precision thermometer deviates from the
currently displayed temperature, you can calibrate the temperature from the control panel.
Example:
• The temperature measured by using the high-precision thermometer is 37.3°C.
• The set point is 37.0°C.
• The temperature currently displayed on the incubator control panel is 37.0°C.
• Calibrate the displayed temperature to be 37.3°C - 0.2°C = 37.1°C.
It is necessary to deduct 0.2°C from the measured temperature to compensate for the
discrepancy between the measured temperature and the actual temperature at the bottom
of the microwell (see also the following NOTE).
• Verify that the temperature measured by the high-precision thermometer settles at 37.2°C
(37.0°C + the 0.2°C difference between the measured temperature and the actual
temperature at the bottom of the microwells).
The display on the incubator control panel will change back from 37.1°C to 37.0°C.