Vitrolife EmbryoScope - Calibrating the O; Concentration

EmbryoScope time-lapse incubator
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5.6.3 Calibrating the O
2
concentration
NOTE
• Attaching a O
2
calibration device with a strong internal pump may interfere with the
internal gas control system inside the EmbryoScope time-lapse incubator. Therefore, do
not use a calibration device that absorbs more than 0.5 l/min.
1. In the set point menu, press the right or the left arrow until the O
2
menu item is
displayed: . (O
2
menu item – the set point is: 5.0).
2. Press and hold the down arrow for three seconds until the display stops flashing
 to enter the calibration menu. When the change of menu is successful, the
display will show .. (O
2
calibration – the calibration is: 5.0).
3. Press and hold the button. The display will now alternate between showing . .
(O
2
calibration – the calibration is: 5.0) and .(calibration – 5.0). This indicates
that you are about to calibrate the O
2
concentration.
4. While still holding the button, press either the up or the down arrow to
increase or decrease the O
2
calibration. The value of the O
2
concentration is adjusted by
0.1% each time you press the up or down arrow once.
You should adjust the O
2
concentration to match the reading on the external gas analyser,
i.e. if the gas analyser reads 5.6%, you need to adjust the value on the display to 5.6.
5. Release the button to save the calibration.
6. Press and hold the up arrow until the display stops flashing  to return
to the set point menu. When the change of menu is successful, the display will show:
. (O
2
menu item – the set point is: 5.0).
7. Wait 10-20 minutes until the O
2
concentration is stable, and then validate the concentration
by following the procedure described in section 5.5.2. You can also monitor the O
2
concentration by pressing the O
2
button on the touch screen of the EmbryoScope time-
lapse incubator.