Vitrolife EmbryoScope - Calibrating the CO; Concentration

EmbryoScope time-lapse incubator
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5.5.3 Calibrating the CO
2
concentration
NOTE
• Attaching a CO
2
calibration device with a strong internal pump may interfere with the
internal gas control system inside the EmbryoScope time-lapse incubator. Therefore, do
not use a calibration device that absorbs more than 0.5 l/min.
1. In the set point menu, navigate to the CO
2
menu item by pressing either the right or the left
arrow until the CO
2
concentration is displayed: . (CO
2
menu item – the
set point is: 5.0).
2. Press and hold the down arrow for three seconds until the displays stops flashing
 to enter the calibration menu. When the change of menu is successful, the
display will show: .. (CO
2
calibration – the calibration is: 5.0).
3. Press and hold the button. The display will now alternate between showing
. . (CO
2
calibration – 5.0) and . (calibration menu is active – the
calibration is: 5.0). This indicates that you are about to calibrate the CO
2
concentration.
4. While still holding the button, press either the up or the down arrow to
increase or decrease the CO
2
concentration. The value of the CO
2
concentration is adjusted
by 0.1% each time you press the up or down arrow once.
You should adjust the CO
2
concentration to match the reading on the external gas analyser,
i.e. if the gas analyser reads 5.6%, you need to adjust the value on the display to 5.6.
5. Release the button to save the calibration.
6. Press and hold the up arrow until the display stops flashing to enter
the calibration menu. When the change of menu is successful, the display will show:
.(CO
2
set point – the set point is: 5.0).
7. Wait 10-20 minutes until the CO
2
concentration is stable, and then validate the
concentration by following the procedure described in section 5.5.2. You can also monitor
the CO
2
concentration by pressing the CO
2
button on the touch screen of the EmbryoScope
time-lapse incubator.