EmbryoScope time-lapse incubator
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• Technical alarm (communicated to the external alarm system):
o The power to the entire EmbryoScope time-lapse incubator is completely cut off.
• Malfunction information signals (by default activated on the EmbryoScope time-lapse
incubator 15 minutes after the error has occurred. Also communicated to the external
alarm system after an additional two minutes):
o Motor errors
o Camera errors
o No response to displayed dialogue box
o Hall sensor error.
• Warning information signals (not communicated to the external alarm system):
o The integrated PC has been in error for more than five minutes.
o The software integrated in the EmbryoScope time-lapse incubator is not running.
o The connection between the incubator and the integrated PC has been interrupted.
o The load door to the embryo chamber has been left open for more than 30 seconds.
o The EmbryoScope time-lapse incubator has been paused for more than one hour
without reinsertion of an EmbryoSlide culture dish.
o The EmbryoScope time-lapse incubator has been left in Check mode for more than
half an hour.
Air flow:
• Recirculation: > 60 l/h (full purification of gas volume every 20 minutes).
• HEPA filter retains 99.97% particles > 0.3 µm.
• Active carbon filter.
Embryo images:
• 1280 x 1024 pixels monochrome CCD camera.
• Leica custom-made, high-quality 20x, 0.40 LWD Hoffman Modulation Contrast objective
providing a resolution of 3 pixels per µm.
• Illumination: single red LED (635 nm, duration < 0.1 seconds per image).
• Total light exposure time: < 50 seconds per day per embryo.
• The images are stored in a resolution of 500 x 500.
• Time between image acquisitions: 10 minutes’ cycle time for a maximum of 7 focal planes,
2 minutes’ cycle time for a maximum of 1 focal plane.
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