1-6 Understanding Operating Principles
Exploring injection principles
The autosampler has three injection modes:
• Full loop– For maximum injection volume precision
• Partial loop needle overfill – For maximum flexibility
• Partial loop – For zero sample loss
Defining pressure-assisted sample aspiration
For all injection modes, the instrument method can enable loop injection with
pressure-assisted sample aspiration. This option pairs an on-board
compressor with intelligent valve switching and highly accurate syringe
control, resulting in these benefits:
• Helps aspirate viscous samples
• Reduces the risk of bubbles in the sample line
• Prevents unwanted sample movement in the needle
Pressurizing vial
Buffer tubing
HPLC pump
Sample loop
HPLC column
Air pressure
Syringe
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