1-8 Understanding Operating Principles
Full loop injections
In the standard configuration, full loop injections yield maximum possible
reproducibility < 0.3% RSD but not necessarily maximum accuracy, because
loop volume is typically specified with an accuracy of ± 10%. Typical sample
usage is 330 µL (3 × loop overfill + 30 µL flush volume for the needle). The
minimum flush volume of 30 µL is recommended; you can program smaller
flush volumes, but they decrease performance.
This is the full loop injection sequence:
1. The injection valve moves to the Inject position, and the sample needle
and air needle enter the well or vial. When the instrument method
enables sample pressurization, the air needle applies headspace
pressure to prevent air or vapor bubbles from forming during sample
aspiration.
Sample
Buffer tubing
Syringe
Needle
Sample loop
INJECT
Column
Pump
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