Waters 2707 - Using Well Plates and Vial Trays

1-24 Understanding Operating Principles
Air segment with partial loop injections
When an air segment has been programmed, it appears at the front of the first
plug of transport liquid and at the front of every sample plug.
The following conditions apply to this injection mode:
• The air segment at the front of the sample plug injects into the HPLC
system
• No headspace pressure can be applied on vials or wells to avoid sample
errors arising from air expansion during exchange from the sample
vial/well to the transport position
Using well plates and vial trays
The instrument accommodates the following well plates and vial holders:
• 96 well tall and deep microtiter (1 or 2 mL)
• 96 well short microtiter (300 µL)
• 384 well short or tall (100 or 250 µL)
• 48 position 2-mL vial holder trays
• 12 position 10-mL vial holder trays (must be used with the prep kit)
Tip: Data-system references to trays 1 and 2 correspond to the left and
right trays, respectively, in the autosampler.
Needle injection loop
Buffer tubing
Sample Eluent Transport
Sample
Eluent Transport Air
Transport
Transport Air
With
air
Without
air