2-10 Preparing the Autosampler
Working with waste tubing
To dispose of general waste liquids
1. Connect the tee end of each drain tubing to the right- and left-hand
drain hose connectors.
Tip: Point the center tee port upward.
2. Place the other ends of the drain tubings in a waste container on the
floor.
Tip: General waste tubing removes all liquid dispensed to the wash
position, as well as uninjected sample liquid and condensate from the
cooler.
Tip: To keep the flow path clear, ensure that the drain or waste tubes
are untwisted.
Accommodating the wash solvent and syringe rinse
To fill the wash solvent tubing
1. Fill a clean wash solvent bottle with wash solvent, and degas it.
Recommendation: Use only water or organic wash solvents, preferably
distilled water and methanol (80%/20%) or mobile phase.
Recommendation: To improve the wash solvent degassing, use a Waters
inline degasser.
Caution: To avoid hindering waste drainage, route the waste tubing
downward, toward the waste container, with no rise in elevation.
Caution: To avoid blockages, which can severely damage the
instrument, do not use crystalline or buffer solutions as wash solvents.
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