Table of Contents vii
1 Understanding Operating Principles ................................................ 1-1
Describing the autosampler ........................................................................... 1-2
Listing component parts.................................................................................. 1-3
Identifying options ........................................................................................... 1-5
Exploring injection principles ....................................................................... 1-6
Defining pressure-assisted sample aspiration............................................... 1-6
Understanding syringe and buffer tubing...................................................... 1-7
Full loop injections........................................................................................... 1-8
Partial loop needle overfill injections ........................................................... 1-13
Partial loop injections.................................................................................... 1-18
Using well plates and vial trays .................................................................. 1-24
2 Preparing the Autosampler ................................................................. 2-1
Preparing for installation ............................................................................... 2-2
Setting up the instrument ............................................................................... 2-2
Plumbing the instrument ................................................................................ 2-3
Identifying instrument tubing ........................................................................ 2-7
Routing and connecting the tubing................................................................. 2-8
Working with waste tubing........................................................................... 2-10
Accommodating the wash solvent and syringe rinse................................... 2-10
Making Ethernet connections ...................................................................... 2-11
Controlling I/O connections ......................................................................... 2-12
Defining TTL inputs...................................................................................... 2-12
Explaining contact closure output ................................................................ 2-12
Table of Contents