Exploring injection principles 1-7
The syringe aspirates the sample from a vial into the sample loop. Buffer
tubing between the syringe and the injection valve prevents syringe
contamination. Wash solvent serves to:
• Remove sample from the buffer tubing and sample needle
• Rinse the buffer tubing and sample needle
Understanding syringe and buffer tubing
The instrument has two syringe sizes:
• 500 µL (standard)
• 2500 µL (included in the prep kit)
With standard 1000-µL buffer tubing and the standard 100-µL sample loop,
injection volume ranges are as follows:
• Full loop – 100 µL
• Partial loop needle overfill – 0 to 50 µL
• Partial loop – 0 to 27 µL
Maximum injection volumes are calculated as follows:
• Full loop – Loop volume
• Partial loop needle overfill – Half the loop volume
• Partial loop – (Loop volume - [3 × needle volume])/2
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