Exploring injection principles 1-13
Partial loop needle overfill injections
In the standard configuration, partial loop needle overfill injections yield
maximum accuracy and reproducibility better than 0.5% RSD for injection
volumes > 10 µL. The minimum flush volume of 30 µL is recommended.
This is the partial loop needle overfill injection sequence:
1. The injection valve moves to the Inject position, and the sample needle
and air needle enter the vial or well. When the instrument method
enables sample pressurization, the air needle applies headspace
pressure to prevent air or vapor bubbles from forming during sample
aspiration.
Sample
Buffer tubing
Syringe
Needle
Sample loop
INJECT
Column
Pump
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