1-12 Understanding Operating Principles
chromatography (HPLC) mobile phase. Sample enters the column, and
analysis starts.
After each injection, a wash routine of programmable volume occurs.
Air segment with full loop injections
A 5-µL air segment that is at the front of the flush volume but not injected can
minimize sample dilution caused by dispersion during aspiration.
With a standard needle, recommended flush volumes are 30 µL for injections
that incorporate air segments and 35 µL for injections that do not. To enhance
performance with highly viscous samples, larger flush volumes and reduced
syringe speeds may be needed.
Sample
Buffer tubing
Syringe
Needle
Sample loop
INJECT
Column
Pump
Needle injection loop
Buffer tubing
Sample Eluent Flush
Sample Eluent Flush Air
With
air
Without
air
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