Waters 2707 - Page 76

4-6 Troubleshooting
If no injection occurs:
Cause Solution
Blockage in flow path 1. Disconnect the needle from the valve.
2. Start a manual wash.
3. If solvent flows from the injection port, inspect
the needle; if no solvent flows from the
injection port, disconnect the buffer tubing
from the injector valve.
4. Start a manual wash.
5. If solvent flows from the open end, inspect the
rotor seal; if not, disconnect the buffer tubing
from the syringe valve.
6. Start a manual wash.
7. If solvent flows from syringe valve, inspect the
buffer tubing; if not, check for overtightened
connections throughout the flow path and
check the syringe valve.
Leakage in the injection
valve
1. Disconnect the needle tubing and buffer
tubing.
2. Connect port 1 to an HPLC pump.
3. Block port 6.
4. Start the pump at a low flow.
5. Observe ports 3 and 4 for leakage.
6. If leakage occurs at ports 3 and 4, inspect the
rotor seal; if not, reinspect with a manual
valve.
Tip: Observe the maximum allowed pressure of
350 psi to prevent valve leakage.