Axiotech and Axiotech
vario
Carl Zeiss
B 40-020 e 06/99 V
Page
3 OPERATION ...................................................................................................................3-3
3.1 Overview of operation and function controls....................................................................3-3
3.1.1 Operation and function controls of the Axiotech 30 .........................................................3-3
3.1.2 Operation and function controls of the Axiotech 100 .......................................................3-4
3.1.3 Operation and function controls of the Axiotech
vario
100....................................................3-5
3.2 Switch on the microscope................................................................................................3-6
3.2.1 Switch on the Axiotech 30 and Axiotech 100 ...................................................................3-6
3.2.2 Switch on the Axiotech
vario
100 (separate SNT 12 V DC, 100 W power unit, stabilized) .......3-6
3.3 Setting the microscope (basic settings) .............................................................................3-7
3.4 Illumination and contrasting techniques ...........................................................................3-7
3.4.1 Setting of reflected-light brightfield for KÖHLER illumination ............................................3-7
3.4.2 Setting of reflected-light darkfield..................................................................................3-10
3.4.3 Setting of reflected-light interference contrast................................................................3-12
3.4.4 Setting of reflected-light polarization contrast ................................................................3-15
3.4.5 Setting of epi-fluorescence.............................................................................................3-17
3.4.6 Change of filters in the reflector slider............................................................................3-21
3.4.7 Setting of transmitted-light brightfield for Köhler illumination.........................................3-22
3.4.8 Setting of transmitted-light polarization contrast ............................................................3-24
3.4.9 Setting of transmitted-light phase contrast.....................................................................3-29
3.4.10 3D Microscopy ..............................................................................................................3-30
3.5 Attachment of photomicrography equipment.................................................................3-31
3.5.1 Attachment of SLR camera, e.g. CONTAX Aria ...............................................................3-31
3.5.2 Attachment of MC 80 DX microscope camera (35 mm format with film cassette 35 mm)3-33
3.5.3 Attachment of MC 80 DX microscope camera (large format with 4"
×
5" large-frame
attachment)...................................................................................................................3-34
3.6 Attachment of videomicroscopy equipment....................................................................3-35
3.6.1 Selection and assembly of adapters for video cameras ....................................................3-36
3.6.2 Attachment of video camera..........................................................................................3-37
3.7 Measuring, counting and comparing techniques using the microscope ...........................3-38
3.7.1 Length measurements using stage micrometer and crossline micrometer ........................3-38
3.7.2 Length measurement using the digital eyepiece measurement device..............................3-39
3.7.3 Determination of microhardness using the MHT-4 microhardness tester..........................3-42
3.7.4 Determination of grain sizes in material structures .........................................................3-43