Carl Zeiss Axiotech and Axiotech
vario
3-8 B 40-020 e 06/99
In reflected-light brightfield, the KÖHLER illumination principle requires the following settings of the
luminous-field diaphragm and the aperture diaphragm:
• Perform steps described in sections 3.2 and 3.3.
• Move reflector slider HD DIC (3-7/5) or HD (3-7/6) in the brightfield position (H mark visible on the
reflector slider).
• Place a high-contrast reflected-light specimen on the microscope stage.
• Swing 10× objective into beam path via knurled ring of the nosepiece.
• Look through the binocular tube ( 3-7/7) to see the preset and adjusted eyepieces (also see sections
2.5 and 2.5.2).
Fig. 3-7 Setting of reflected-light brightfield