Axiotech and Axiotech
vario
Carl Zeiss
B 40-020 e 06/99 3-19
3.4.5.2
Setting of epi-fluorescence using the 3 FL reflector slider
• Perform steps described in sections 3.2 and 3.3.
• The 3 FL reflector slider (3-17/3) replaces the reflector slider HD or HD DIC.
☞
The reflector sliders HD and HD DIC can only be pulled out if one of the dust caps ( 3-19/5 or
3-17/2) has been removed from the left or right side of the stand. To do this, unscrew, the two
hexagonal screws (3-19/6) from one side using the SW 3 ball-headed screwdriver, remove the
dust cap and pull out the reflector slider very carefully, since a darkfield component projects
from the reflector slider.
• Attach removed lateral dust cap again.
• Move filter slider (3-17/1) to free light path position.
• In the center position of the 3 FL reflector slider, select the required specimen spot in reflected-light
brightfield and refocus on it via the coarse/fine drive, if required.
• Move the user-specific fluorescence filter combinations in the beam path until the left or right stop
position of the 3 FL reflector slider.
• Open the aperture diaphragm completely.
Fig. 3-17 Setting of epi-fluorescence using the 3 FL reflector slider