Axiovert 40
B 46-0031 e 11/03 2Ć11
2.4 Illumination and contrast techniques
The description/application of illumination/contrast techniques is based on the following microscope setĆ
tings:
• The Axiovert 40 microscope has been switched on.
• The brightness control (2Ć14āā/ā5) has been turned to mid-position.
• The observation/camera path selector switch (2Ć11ā/ā7) has been set to observation.
2.4.1 Use of condensers
Condenser
0.2 0.4 0.55
Working distance
between condenser and
stage surface
wĂ90 mm wĂ53 mm wĂ31 mm
plus 6 mm
vertical travel
Objective magnification 5x, 10x, 20x 5x, 10x, 20x, 32x, 40x 5x, 10x, 20x, 32x, 40x
Microscopic technique Brightfield
phase contrast
Brightfield
phase contrast
VAREL
(10x up to 32x)
PlasDIC
Brightfield
phase contrast
VAREL
PlasDIC
• To fully illuminate object fields even when low-power objectives (< 5x) are used, push condenser (2Ć14ā/ā3)
out of its work position (dashed line in Fig. 2Ć14).
• This position of the condenser is also required when particularly high culture vessels are used in order to
increase the free working distance to approx. 190 mm.
NOTE Only simple survey illumination is obtained, if no condenser is used!
• When using the condenser again for illumination, move it exactly up to the stop position.
NOTE Loosening the three screws (2Ć14ā/ā7) of the condenser changer permits the condenser 0.4 to
be replaced by the condenser 0.2 or 0.55. In doing so, make sure to hold the condenser.
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