Zeiss Axiovert 40 - Plasdic; Observation with Plasdic

Axiovert 40
B 46-0031 e 11/03 2Ć17
2.4.5 PlasDIC
PlasDIC is an innovative interference contrast technique that can be used on the Axiovert 40 CFL in combinaĆ
tion with the following components: Quintuple nosepiece with slot for PlasDIC slider, slider Ph, H, PlasDIC,
slit-diaphragm 5 mm for PlasDIC, 3-position reflector slider P&C with analyzer moduleĂD P&C.
PlasDIC provides a relief-like image and can be used especially with thick specimens. The contrast is variably
adjustable. The wells of microtiter plates can be contrasted up to the edge. It is not necessary to use cultivation
vessels with glass bottom.
• Prepare the microscope as described in Sections 2.4. and 2.4.1.
• Equip the 3-position reflector slider P&C with the analyzer module D P&C (for that, refer to Section 3.2Ă(4)).
• Equip the slider Ph, H, PlasDIC with the slit-diaphragm 5 mm for PlasDIC (see next page).
• Remove the factory-fitted protective slider from the slot on the nosepiece and push the PlasDIC slider
(2Ć22ā/ā4) appropriate for the used objective (A-Plan 10x, LD A-Plan 20x, 32x or 40x) into the slot.
• Fully open the aperture diaphragm (2Ć22ā/ā1) on the condenser (2Ć22ā/ā2).
• Insert the specimen with the appropriate mounting frame in the holding clips (2Ć22ā/ā7) of the attachable
object traverser M.
Fig. 2Ć22 Observation with PlasDIC
1
2
3
4
5
6
7