Chapter 5 Analyze the Relative Standard Curve Experiment
View the Amplification Plot
87
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
Notes
4. View the baseline values (Plot Settings):
a. In the Graph Type drop-down list, select Linear.
b. Select the Baseline check box to show the start cycle and end cycle.
c. Verify that the baseline is set correctly: The end cycle should be set a few
cycles before the cycle number where significant fluorescence is detected. In
the example experiment, the baseline is set correctly.
4b
3c
3a 4a 3b
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