1290 Infinity Quaternary Pump User Manual 77
Using the Pump
4
Solvent Information
Solvent Handling
Handling of Buffers
The following recommendations should be observed when using buffer
solutions:
• Buffers and aqueous solutions are possible sources of algae
contamination, for avoiding related problems, please read “Algae Growth
in HPLC Systems” on page 54.
• For buffer concentrations of 0.1 M or higher using the seal wash option
is strongly recommended.
• Filter buffer solutions to avoid increased wear or blockages that are
caused by undissolved crystals. Always use solvent inlet filters.
• Avoid conditions where mixing of buffers and organic solvents may
cause precipitation, as this impairs the reproducibility of
chromatographic experiments and may also reduce the system life time.
For example in reversed phase chromatography, avoid buffers (especially
phosphate buffers) with a concentration higher than 20 mmol/L. For
phosphate buffers, avoid compositions containing more than 65 %
acetonitrile or other organic solvents.
• Use a minimum flow rate of 5 µL/min or 1 % composition per solvent
channel (whatever is greater) to avoid cross- flow. Cross- flow can be
caused by micro leaks in pump heads and can result in buffer
precipitation in pump heads, channel blocking, or reduced pump head
life time through wear of seals and pistons.
• When installing tubing connections to the MCGV, use lower channels
(A/D) for aqueous solvents and upper channels for organic solvents.
This will re- dissolve precipitates more easily.
• Never leave buffers in a system without flow. Before shutting down a
system, flush it extensively with warm water to avoid clogging of valves,
capillaries, or flow cells or reducing the life time of your column. If the
system is not used for some time, for example more than a day
depending on lab temperature, fill all solvent lines with organic solvent
or water with at least 10 % isopropanol.
• Regularly maintain the LC system.
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