1290 Infinity Quaternary Pump User Manual 83
How to Optimize the Performance of Your Module
5
How to Configure the Optimum Delay Volume
How to Configure the Optimum Delay Volume
The design of the 1290 Infinity Quaternary Pump offers a strongly
decreased delay volume compared to standard 600 bar pressure pumps.
For the 1290 Infinity Quaternary Pump, mixing is done in the
multi- channel gradient valve at ambient pressure. As all pump parts in
the flow path after mixing contribute to the delay volume, this includes
also pump heads of the quaternary pump, flow connections, filters, mixers
etc. Therefore the delay volume of a quaternary pump is by design larger
than that of a binary pump.
All listed components including the inlet weaver and pump heads ensure a
good mixing performance resulting in excellent composition precision and
accuracy, highly reproducible retention times and low baseline noise. This
ensures best results for most applications.
Per default, the 1290 Infinity Quaternary Pump does not require and
include a Jet Weaver, as solvents are mixed in the MCGV and mixing is
further improved in the inlet weaver, pump heads and subsequent parts in
the flow path. Therefore, no Jet Weaver is required for most applications.
The V380 Jet Weaver high performance mixer is optionally available for
demanding applications, which use solvents in different channels (for
example A versus B), that differ strongly in their UV/Vis absorption, for
example by using trifluoroacetic acid (TFA) as a modifier, which has a
high absorbance. Solvent packages created by the pump may persist until
the solvent reaches the detector flow cell. Absorption fluctuations can
then show up as baseline noise, also referred to as mixing noise.
Applications like impurity quantitation or lowest level compound detection
require minimizing this noise. The V380 Jet Weaver strongly improves
mixing and therefore reduces baseline noise and improves sensitivity in
detection. Patented Agilent microfluidic technology offers high mixing
performance at a low internal volume of 380 µL, which is the physical
volume of all channels. It contributes with 150 µL to the pump delay
volume (< 350 µL without Jet Weaver), which is the partial mixer volume
that creates a composition change corresponding to the delay volume.
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