1290 Infinity Quaternary Pump User Manual 53
Using the Pump
4
Preparing the Pump
Preparing the Pump
For best performance of the pump:
• Place solvent cabinet with the solvent bottles always on top (or at a
higher level) of the pump.
• For optimum performance, always use the built- in degasser.
• When using the pump with vacuum degassing unit, flush the degassing
unit with at least 5 mL per channel before operating the pump,
especially when the pumping system had been turned off for a certain
length of time (for example, overnight) and volatile solvent mixtures are
used in the channels.
• Prevent blocking of solvent inlet filters (never use the pump without
solvent inlet filters). Growth of algae should be avoided, see “Algae
Growth in HPLC Systems” on page 54.
• Check pump outlet filters and column frit in regular time intervals. A
blocked pump outlet filter can be identified by black, yellow or greenish
layers on its surface.
• When using buffer solutions, flush the system with water before
switching it off.
• The optional seal wash function should be used when buffer solutions
with concentrations of 0.1 M or higher are being pumped for long
periods of time.
• Never leave an unused pump with water in a channel for an extended
period of time (2-3 days). Always flush with organic solvent or add
10 % isopropanol to water.
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