Checklists 3
Hardware Installation 55
❒ Manual tune explained
❒ View Tunes utility demonstrated and its importance explained
❒ PCI Autotune performed (if applicable)
❒ NCI Autotune performed (if applicable)
❒ Optimization of reagent gas and application dependency on gas flow, source
temperature, and emission current discussed
❒ Importance of retaining tuning and maintenance records discussed
❒ Importance of backing up data, including tune files
Methods
❒ Edit entire method demonstrated with OFN_SN.M, explaining the GC and
MS Scan Parameters.
❒ Method save demonstrated
Data acquisition
❒ Analytical method developed to run both scan and SIM
❒ Steps involved in acquiring data and editing an entire method described
❒ File designated to store the data; data file naming demonstrated
❒ Acquisition parameters saved as part of a method
❒ Use Scan/SIM (amu range 50:300/amu ion 272) on OFN for acquisition
❒ Snapshot demonstrated
Data analysis
❒ Screen layout explained (TIC, spectral windows, command line, title bar,
etc.)
❒ Loading data files demonstrated
❒ TIC, spectra, and extracted ion profiles demonstrated
❒ Integration performed and explained
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