Applied Biosystems QuantStudio 7 Flex - Page 55

Block Plate
96-well 0.1-mL block
QuantStudio
™
3/5 Spectral Calibration Plate 1, (FAM
™
, VIC
™
, ROX
™
, and SYBR
™
dyes), 96-well,
0.1 mL
QuantStudio
™
3/5 Spectral Calibration Plate 2 (ABY
™
, JUN
™
, MUSTANG PURPLE
™
dyes),
96‑well Fast (0.1‑mL) Plate
QuantStudio
™
3/5 Spectral Calibration Plate 3 (TAMRA
™
, NED
™
and Cy
®
5 dyes)
96‑well Fast (0.1‑mL) Plate
384-well block
QuantStudio
™
5 Spectral Calibration Plate 1, (FAM
™
, VIC
™
, ROX
™
TAMRA
™
, and SYBR
™
dyes),
384-well
QuantStudio
™
5 Spectral Calibration Plate 2 (ABY
™
, JUN
™
, MUSTANG PURPLE
™
, NED
™
, and
Cy
®
5 dyes), 384-well
• Centrifuge with plate adapter
•
Powder-free gloves
• Safety goggles
Prepare the plate
IMPORTANT! Before performing a dy
e calibration, you must perform an ROI
calibration, a background calibration, and a uniformity calibration.
IMPORTANT! W
ear powder-free gloves and safety glasses when you prepare the
plate.
1.
Remov
e the dye calibration plate from the freezer, then thaw it at room
temperature for approximately 30 minutes.
IMPORTANT! Use the calibration plates within 2 hours of defrosting them. Until
you are ready to run the plates, store them in the dark and at ambient
temperature (15–30℃). Do not remove the plates from their packaging until you
are ready to run them.
2.
Before using each dy
e plate, vortex the plate for 5 seconds, centrifuge it for
2 minutes at < 1500 rpm, then conrm that the liquid in each dye plate is at the
boom of the wells. If not, centrifuge the plate again at a higher rpm and for a
longer period of time.
Chapter 2 Calibrate the instrument
Dye calibration
2
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
55