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Applied Biosystems QuantStudio 7 Flex User Manual

Applied Biosystems QuantStudio 7 Flex
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Block Plate
96-well 0.1-mL block
QuantStudio
3/5 Spectral Calibration Plate 1, (FAM
, VIC
, ROX
, and SYBR
dyes), 96-well,
0.1 mL
QuantStudio
3/5 Spectral Calibration Plate 2 (ABY
, JUN
, MUSTANG PURPLE
dyes),
96well Fast (0.1mL) Plate
QuantStudio
3/5 Spectral Calibration Plate 3 (TAMRA
, NED
and Cy
®
5 dyes)
96well Fast (0.1mL) Plate
384-well block
QuantStudio
5 Spectral Calibration Plate 1, (FAM
, VIC
, ROX
TAMRA
, and SYBR
dyes),
384-well
QuantStudio
5 Spectral Calibration Plate 2 (ABY
, JUN
, MUSTANG PURPLE
, NED
, and
Cy
®
5 dyes), 384-well
Centrifuge with plate adapter
Powder-free gloves
Safety goggles
Prepare the plate
IMPORTANT! Before performing a dy
e calibration, you must perform an ROI
calibration, a background calibration, and a uniformity calibration.
IMPORTANT! W
ear powder-free gloves and safety glasses when you prepare the
plate.
1.
Remov
e the dye calibration plate from the freezer, then thaw it at room
temperature for approximately 30 minutes.
IMPORTANT! Use the calibration plates within 2 hours of defrosting them. Until
you are ready to run the plates, store them in the dark and at ambient
temperature (15–30). Do not remove the plates from their packaging until you
are ready to run them.
2.
Before using each dy
e plate, vortex the plate for 5 seconds, centrifuge it for
2 minutes at < 1500 rpm, then conrm that the liquid in each dye plate is at the
boom of the wells. If not, centrifuge the plate again at a higher rpm and for a
longer period of time.
Chapter 2 Calibrate the instrument
Dye calibration
2
QuantStudio
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
55

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Applied Biosystems QuantStudio 7 Flex Specifications

General IconGeneral
BrandApplied Biosystems
ModelQuantStudio 7 Flex
CategoryLaboratory Equipment
LanguageEnglish

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