Applied Biosystems QuantStudio 7 Flex - Perform the Dye Calibration

IMPORTANT! The software guides you through the calibration of each dye
separately. You must set up, run, and analyze each dye independently.
1.
In the QuantStudio
™
Real-Time PCR Softw
are Home tab, click Instrument
Console.
2.
In the Instrument Console, select the icon for your instrument, then click
Manage Instrument.
Note: If the Manage Instrument buon is inactive, add your system to the My
Instruments group.
3.
In the Instrument Manager, start the calibration:
a.
Click
Maintenance, then click Dye.
b.
In the Dye Calibration screen, select System Dye Calibration, then click
Start Calibration.
4.
In the Dye Calibration screen, select the dy
e to calibrate from the Dye Name
drop-down list, then perform the calibration as instructed.
5.
At the boom of the Setup tab, enter the reagent information for the dye plate or
array card that you are using.
6.
Load the dy
e plate or array card into the instrument:
a.
From the instrument touchscreen, tap
to eject the instrument tray.
P
erform the dye
calibration
Chapter 2 Calibr
ate the instrument
Dye calibration
2
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QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide