Applied Biosystems QuantStudio 7 Flex - CHAPTER 2 Calibrate the instrument

Calibrate the instrument
■
Calibration overview ................................................. 27
■
Enable or change the calibration reminders .............................. 32
■
Guidelines for preparing calibration plates and array cards ................ 36
■
Preparing array cards for instrument calibration .......................... 37
■
ROI calibration ....................................................... 41
■
Background calibration ............................................... 45
■
Uniformity calibration ................................................ 49
■
Dye calibration ....................................................... 53
■
Normalization calibration (TaqMan
®
Array Card only) .................... 59
■
Verify the instrument performance ..................................... 62
■
Troubleshooting ...................................................... 72
■
How to identify contamination ......................................... 81
■
Remove a plate stuck within the instrument ............................. 81
IMPORTANT! Perform the procedures for preparing array cards only if you are
verifying the performance of a QuantStudio
™
7 Flex System with an array card sample
block. The QuantStudio
™
6 Flex System does not support the array card sample block.
2
26
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide