The QuantStudio
™
6 and 7 Flex Systems collect raw uorescence data at dierent
points during a PCR, depending on the type of run that the
QuantStudio
™
6 and 7
Flex Systems perform:
Table 1
Run type Data collection point
Real-time
runs
Standard curve The QuantStudio
™
6 and 7 Fl
ex Systems collect
data following each extension step of the PCR.
Relative standard
curve
Comparative C
t
(ΔΔC
t
)
Melting curve
Post-PCR
(endpoint)
runs
Genotyping The QuantStudio
™
6 and 7 Flex Systems collect
data:
1. Before the PCR. (For presence/absence
experiments, data collection before the
PCR is optional, but recommended.)
2. (Optional) During the PCR. The
QuantStudio
™
6 and 7 Flex System can
collect data during the run (real-time);
collecting data during the run can be
helpful for troubleshooting.
3. After the PCR.
Presence/absence
The instrument collects raw uorescence data following each extension step of the
PCR. A data collection point, or read, on the QuantStudio
™
6 and 7 Flex System
consists of three phases:
Data collection
Chapter 1 Getting started
About the QuantStudio
™
6 and 7 Flex Real-Time PCR Systems
1
14
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
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