Applied Biosystems QuantStudio 7 Flex - Guidelines for Preparing Calibration Plates and Array Cards

Figure 4 Calibr
ation reminders screen for the instrument with a TaqMan
®
Array Card block
Guidelines for preparing calibration plates and array cards
Observe the following guidelines when preparing calibration plates and array cards:
• Wear appropriate protective eyewear, clothing, and gloves.
• Prepare and run calibration plates and array cards within the recommended time
limits listed below.
Consumable Time to thaw
After thawed,
run within…
All 96-/384-well calibration consumables
[1]
30 minutes 120 minutes
All array card calibration consumabes
[2]
30 minutes 120 minutes
RNase P plate (96-/384-well) 5 minutes 30 minutes
RNase P array card
[3]
15 minutes 60 minutes
[1]
A
ll 96-well plates and 384-well plates used in the calibration of the instrument (ROI, background,
uniformity, and dye).
[2]
All array cards used in the calibration of the QuantStudio
™
7 Flex System only (ROI, background,
uniformity, dye, and normalization).
[3]
For use with the QuantStudio
™
7 Flex System only.
• (Plates only) Do not discard the packaging for the calibration plates. Each plate
can be used to calibrate the QuantStudio
™
6 and 7 Flex Systems 3 times for up to
6 months if it is stored in its packing sleeve.
Chapter 2 Calibr
ate the instrument
Guidelines for preparing calibration plates and array cards
2
36
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide