Applied Biosystems QuantStudio 7 Flex - Glossary

Glossary
Glossary
Plot of the re-scaled melt curve.
In a diploid organism, one of two DNA sequences found at the same locus (for
example, a particular gene), but located on homologous chromosomes. Two
corresponding alleles may have the identical sequence, or they may dier somewhat,
often at one or more single-base sites (SNPs).
A segment of DNA amplied during PCR.
Part of the instrument run in which PCR amplies the target. Fluorescence data
collected during amplication are displayed in an Amplication Plot, and the data are
used to calculate results.
Note: Only quantitative real-time PCR experiments, not end-point experiments, take
amplication data into account.
Calculation of the eciency of the PCR amplication in an experiment. EFF% is
calculated using the slope of the regression line in the standard curve. A slope close to
–3.32 indicates optimal, 100% PCR amplication eciency.
Display of data collected during the cycling stage of PCR amplication. The
Amplication Plot can be viewed as:
• Baseline-corrected normalized reporter (ΔR
n
) vs. cycle
• Normalized reporter (R
n
) vs. cycle
• Threshold cycle (C
t
) vs. well
Part of the instrument run in which PCR amplies the target. The amplication stage,
called a cycling stage in the thermal prole, consists of denaturing, primer annealing,
and extension steps that are repeated. Fluorescence data collected during the
extension stage are displayed in an amplication plot, and the data are used to
calculate results. With chemistry, the last two steps of a PCR stage are typically
combined.
See also “cycling stage“ on page 185.
In a PCR reaction mix, two target-specic primers or two primers and a probe used to
amplify a target.
aligned melt curve
plot
allele
amplicon
amplification
amplification
efficiency (EFF%)
Amplification Plot
amplification
stage
assay
184
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide