Applied Biosystems QuantStudio 7 Flex - Page 185

PCR reaction component in assays. The assay mix contains primers designed to
amplify a target and a probe designed to detect amplication of the target.
An analysis seing in which the softw
are calculates the baseline start and end cycles
for the amplication plot.
See also “baseline“ on page 185.
An analysis seing in which the software calculates the baseline start and end cycles
and the threshold in the amplication plot. The software uses the baseline and
threshold to calculate the threshold cycle (C
t
).
See also “threshold cycle (Ct)“ on page 193.
Type of calibration in which the instrument performs reads of a background plate or
array card, averages the spectra recorded during the run, and extracts the resulting
spectral component to a calibration le. The software then uses the calibration le
during subsequent runs to remove the background uorescence from the run data.
From the Amplication Plot, a cycle-to-cycle range that denes background
uorescence.
The magnitude of normalized uorescence signal generated by the reporter. In
experiments that contain data from real-time PCR, the magnitude of normalized
uorescence signal generated by the reporter at each cycle during the PCR
amplication. From the ΔR
n
vs. Cycle Amplication Plot, ΔR
n
is calculated at each
cycle as:
ΔR
n
(cycle) = R
n
(cycle) -R
n
(baseline), where R
n
= normalized reporter
See also “normalized reporter (Rn)“ on page 188.
In presence/absence experiments, a reaction that contains IPC blocking agent, which
blocks amplication of the internal positive control (IPC). In QuantStudio
™
Real-Time
PCR Software, also the name of the task for the IPC target in wells that contain IPC
blocking agent. See also “negative control-blocked IPC wells“ on page 187.
The CE Mark (an acronym for the French “Conformite Europeene”) of conformity
certies that a product has met the essential requirements of the applicable European
Directive.
See “reagents“ on page 189.
Sample of known genotype or melt-curve shape.
See “threshold cycle (Ct)“ on page 193.
The QuantStudio
™
Real-Time PCR Software uses the Baseline Threshold algorithm.
See “threshold cycle (Ct)“ on page 193.
assay mix
automatic
baseline
automatic
thr
eshold
background
calibration
baseline
baseline-corrected
normalized
reporter (ΔR
n
)
blocked IPC
CE mark
chemistry
control
C
t
C
t
algorithm
cycle threshold
Limited product warranty
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
185