Applied Biosystems QuantStudio 7 Flex - Create a Background Plate; Create a Background Array Card (Quantstudio 7 Flex System Only)

IMPORTANT! Wear powder-free gloves while creating the background plate.
1.
Remov
e a MicroAmp
™
96/384-Well Optical Reaction Plate from its box and place
it on a clean, dry surface.
2.
Aliquot 20 µL deionized water to each well of the reaction plate.
3.
Seal the plate using an optical adhesive cover or optical at caps.
4.
Use the plate for background calibration as you would a background plate from
the spectral calibration kit.
1.
Remove an array card from its box and place it on a clean, dry surface.
2.
Using a permanent marker, write
“Background” on the side of the
empty card.
3.
Pipet 100 µL of deionized water into
each of the eight reservoirs in the
card:
a.
Place the array card on a lab
bench, with the foil side down.
b.
Load 100 µL of the solution into a pipee.
c.
Hold the pipee in an angled position (∼45°) and place the tip into the ll
port.
There is a ll port on the left arm of each ll reservoir – the larger of the two
holes.
GR2158
Microfluidic Card
Card Ports detail
21
1
Fill port
2
Vent port
Create a
background plate
Create a
back
ground array
card
(QuantStudio
™
7
Flex System only)
Mark
here
Appendix F Calibration consumable preparation
Create a background plate or array card
F
152
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide