Applied Biosystems QuantStudio 7 Flex User Manual

Part of the run method that species the temperature, time, ramp, and data collection
points for all steps and stages of the instrument run.
experiment document le (.eds)
The Thermo Fisher Scientic name for the electronic records that comprise all
information about a particular plate or array card, including metadata (name,
barcode, comments), plate setup (w
ell contents, assay denitions), run method
(thermal cycling protocol), run results, analysis protocol, analysis results, audit
records, and other consumable-specic data. Experiment document les have the .eds
sux.
In Amplication Plots, the level of uorescence above the baseline and within the
exponential growth region.
The PCR cycle number at which the uorescence meets the threshold in the
Amplication Plot. See also “threshold“ on page 193 and “Amplication Plot“ on
page 184.
See “melting temperature (Tm)“ on page 187.
Instrument display that you touch to control the instrument.
Type of calibration in which the software measures sample block uniformity. The
calibration generates data that compensate for the physical eects of the
QuantStudio
™
6 and 7 Flex Systems lters on data collected during a experiment.
From the software, the task for the target in wells that contain the sample being tested.
In quantication experiments, the task for the target in wells that contain a sample
with unknown target quantities. In genotyping experiments, the task for the SNP
assay in wells that contain a sample with an unknown genotype. In presence/absence
experiments, the task for the target in wells that contain a sample in which the
presence of the target is not known.In melt curve experiments, the task for the target
in wells that contain a sample with an unknown melt curve prole.
A sample (or group of samples) with a unique melt curve (that is, the melt curve is
dierent from the melt curves of other samples or controls used in the experiment).
The software determines melt curve dierences by the melting temperature (T
m
) and
the shape of the melt curve.
From the standard curve, the value of y where the regression line crosses the y-axis.
The y-intercept indicates the expected threshold cycle (C
t
) for a sample with quantity
equal to 1.
thermal profile
threshold
threshold cycle
(C
t
)
T
m
touchscreen
uniformity
c
alibration
unknown
variant
y-intercept
Limited product warranty
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
193

Applied Biosystems QuantStudio 7 Flex Specifications

General

Brand	Applied Biosystems
Model	QuantStudio 7 Flex
Category	Laboratory Equipment
Language	English