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Applied Biosystems StepOne User Manual

Applied Biosystems StepOne
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DRAFT
September 25, 2007 1:07 am, 4376782_Maintain_the_System.fm
Chapter 6 Maintain the System
Regular Maintenance
99
Applied Biosystems StepOne
and StepOnePlus
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
Notes
How to Create a
Background Plate
Whenever possible, use a background plate included with the spectral calibration kit. The
background plate contains a buffer that accurately models the reagents used for PCR, and
produces high-quality calibration data. However, if a background plate from a spectral
calibration kit is not available, you can create one as explained below.
Materials Required
Deionized water
MicroAmp Optical Adhesive Film or MicroAmp
8-Cap Strip (Flat Caps only)
•MicroAmp
Optical Reaction Plate
Pipettor, 200-µL and pipette tips
Powder-free gloves
Safety glasses
Create the Background Plate
IMPORTANT! Wear powder-free gloves while creating the background plate.
1. Remove a reaction plate from its box and place it on a clean, dry surface.
2. Pipette 30 µL of deionized water to each well of the reaction plate.
3. Seal the reaction plate using an optical adhesive film or optical flat caps.
4. Use the reaction plate to perform the background calibration.

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Applied Biosystems StepOne Specifications

General IconGeneral
BrandApplied Biosystems
ModelStepOne
CategoryLaboratory Equipment
LanguageEnglish

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