Applied Biosystems StepOne User Manual

DRAFT September 25, 2007 1:07 am, 4376782_Maintain_the_System.fm
Chapter 6 Maintain the System
Infrequent Maintenance
119
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
Notes
Move and
Reconnect the
Instrument
PHYSICAL INJURY HAZARD. Do not attempt to lift the
instrument or any other heavy objects unless you have received related training. Incorrect
lifting can cause painful and sometimes permanent back injury. Use proper lifting
techniques when lifting or moving the instrument. At least two people are required to lift
the instrument.
IMPORTANT! Moving your instrument can create subtle changes in the alignment of the
instrument optics. Applied Biosystems recommends that you run a TaqMan
®
RNase P
Fast Instrument Verification Plate after you move the instrument to confirm the
performance of the system.
1. Move your instrument according to the guidelines in step 6 on page 16.
Do not carry the instrument by the instrument drawer. Carrying
the instrument by the drawer can damage the instrument optics.
2. Install the components of the system according the chosen layout.
If the RNase P experiment performed at the end of the installation:
• Passes, then you have successfully moved the instrument.
• Fails, recalibrate the instrument:
a. Perform a spatial calibration (see “Perform a Spatial Calibration” on page 90).
b. Perform a background calibration (see “Perform a Background Calibration” on
page 94).
c. Perform a dye calibration (see “Perform a Dye Calibration” on page 100).
d. Perform another RNase P experiment (see “Perform the RNase P Experiment”
on page 38 or page 60).
Layout Description
Perform the installation
described in…
Colocated In this layout, the instrument is connected to
the colocated computer by the yellow
StepOne system cable.
Chapter 3, “Install the
Colocated Layout,” on
page 25
Standalone In this layout, the instrument is not directly
connected to the computer running the
StepOne
™
software.
Chapter 4, “Install the
Standalone Layout,” on
page 49
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Applied Biosystems StepOne Specifications

General

Brand	Applied Biosystems
Model	StepOne
Category	Laboratory Equipment
Language	English