158
User’s Manual
CellaVision
®
DM1200
(Cont'd)
(Cont'd)
a May Grünwald stain, stock solution: Merck M1424 Eosin-methylenblue
solution, modified for microscopy
(contains methanol).
Store at +15 ºC to +25 ºC.
b May Grünwald working solution: Dilute 1 part of May Grünwald stock
solution with 1 part buffer work solution.
Stable for 8 hours.
c Buffer stock solutions 1 and 2: 1) 9.07g KH
2
PO
4
(0.067M) ad 1000ml
deionized water
Store at +4 ºC to +8 ºC.
2) 9.45 g Na
2
HPO
4
(0.067M) ad
1000ml deionized water
Store at +4 ºC to +8 ºC.
d Buffer working solution, pH 6.8 Combine 127 ml KH
2
PO
4
stock
solution with 123 ml Na
2
HPO
4
stock
solution ad 5000 ml deionized water.
Adjust to pH 6.8.
Store at +4 ºC to +8 ºC.
Durable for a month at +4 ºC.
e Giemsa stain, stock solution: Merck M9204 Azur-eosine-
methylenblue solution for microscopy
(contains methanol).
Store at +15 ºC to +25 ºC.
Durable for several months if kept in a
dark bottle.
f Giemsa working solution: Dilute 1 part Giemsa stock solution with
19 parts buffer work solution.
Stable for 8 hours.
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