25
User’s Manual
CellaVision
®
DM1200
3 Quality Control
3.1 Cell Location Performance
The Cell location test is used to verify the slide preparation process and the system
hardware. The cell location performance shall be verified at regular intervals and
after changes in staining procedure or staining solutions by running the Cell
location test.
Note! Running the test once or twice a day is a recommended interval at the high-
load laboratory.
3.1.1 Cell Location for Peripheral Blood
The test establishes how many percent of the nucleated cells (i.e., WBC and
NRBC) that are found on a slide.
The Cell Location tool may be accessed in the Tools menu.
Slide Requirements
Use a freshly stained blood sample with a WBC count in the normal WBC range.
To reduce processing time, a WBC count above 7x10
9
/l is recommended. If the
system cannot locate at least 100 nucleated cells, the result will be discarded.
The percentage of non-nucleated cells (i.e., all other objects that are not identified
as being a WBC or an NRBC, e.g. smudge cells) must not exceed 30% of the total
number of objects.
The slides must have a barcode label, starting with the text "QC". All slides with
this kind of label are automatically treated as cell location test slides. When the
slide has been processed it will only be available in the Cell Location tool. Pre-
printed QC labels are available from your local dealer.
Running a Cell Location Test
1. Select a slide that meets the requirements and put a QC label on it.
2. Put the slide in a magazine, place it in the DM and process the slide.
3. Go to the Cell Location tool when the slide has finished.
4. Select the new slide at the top of the slide list.
5. Go through all the images belonging to the slide and examine them for missed
nucleated cells. Enter the number of missed cells, if any, in the input field for
each image.
6. When all images have been examined the result will appear at the end. Check
that it is within acceptable limits.
(Cont'd)
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