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Quick Setup Guide

Create and Load the Liberty Method

NOTE

To load an existing method, skip to step 9.

Create the Method

1. Open the appropriate folder or sub folder in the Methods box for the new method.

2. Click the New Method button.

3. The method will appear in the appropriate folder. Enter a name for the method and press Enter.

4. Click into the Sequence box. A list of the amino acids in the current bottle setup will drop down. To enter the

sequence, either click the appropriate amino acid positions in the drop-down, or type the one-letter abbreviation

for the desired amino acids.

NOTE

The amino acids in the sequence should be entered from N-terminus to C-terminus.

5. Select the scale (0.005 mmol to 5.0 mmol) from the Synthesis Scale box, then select the resin type (Standard

or High Swelling) from the Resin Type box.

NOTE

The scale and resin type must be selected before changing any of the other parameters, as each scale

and resin type has specic default options that will be loaded upon selection.

6. The remaining options can now be selected.

6.1. C-Terminus: The C-terminus (acid or amide) is only used to calculate the molecular weight, and does

not affect the synthesis conditions.

6.2. Resin Type: The resin type will determine the rst coupling step of the reaction. If Preloaded is

checked, the software assumes the C-terminal amino acid is already loaded on the resin and

therefore skips that coupling. If Not Preloaded is selected, the rst coupling will proceed as normal.

If Chloride Loading is selected, a chloride coupling step will be used for the C-terminal amino acid

coupling.

6.3. Resin Name: The resin name entered in this box will appear in the Method Report, but will not affect

the synthesis conditions.

6.4. Resin Loading: The substitution value entered in this box (in meq/g) will appear in the Method

Report, but will not affect the synthesis conditions.

6.5. Resin Cycle: The resin cycle determines the swelling conditions used to prepare the resin at the

beginning of the synthesis. Make sure the amino acid cycles match the Resin Swelling Cycle (HS

vs. non HS).

6.6. Final Deprotection Cycle: By default, the N-terminal protecting group is removed at the end of the

synthesis. Selecting an alternate Final Deprotection cycle allows control of this step.

6.7. Bottle Setup: If using a bottle setup other than the standard default, it must be selected prior to

entering the sequence.

CEM Liberty Blue User Manual

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