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Quick Setup Guide
1.2.2 Other Reagents: For the other reagents, click on the appropriate tab (Activator, Activator Base,
Deprotection) and then select the appropriate reagent from the list. Enter the concentration
and nal volume needed, and the calculator will update with the mass required.
1.2.3 If desired, print a Reagent Report.
Load the Reagents onto the Liberty Blue
1. Load all reagents onto the Liberty Blue.
1.1. Connect centrifuge tubes with amino acid solution to the appropriate positions. Connect empty
centrifuge tubes to all unused positions.
1.2. Connect activator and activator base bottles to the appropriate positions on the system.
1.3. Check the level of solvent remaining in the solvent bottle. If more solvent is required, use the Change
Bottle Main Solvent operation to replace the bottle (see Change Bottle Main Solvent in Section 4).
1.4. Check the level of deprotection solution remaining in the deprotection bottle. If more deprotection
is required, use the Change Bottle Deprotection operation to replace the bottle (see Change Bottle
Deprotection in Section 4).
Load the Resin into the Reaction Vessel
1. Load the resin (100-200 mesh only) into the reaction vessel.
CAUTION
The 30 mL reaction vessel should be used for 0.005 - 0.25 mmol syntheses (up to 1 g of resin). The 125
mL reaction vessel should be used for 0.5 - 5 mmol syntheses (up to 8 g of resin).
1.1. Remove the reaction vessel from the microwave cavity.
1.2. Unscrew the bottom port of the beige union connector on the vessel drain line. Then, unscrew the
vessel body from the attenuator.
1.3. Weigh the appropriate amount of dry resin (100-200 mesh only) into the vessel body.
1.4. Connect the vessel body to the attenuator, then connect the drain line to the vessel body using the
union connector tting.
1.5. Place the vessel back into the microwave cavity, securing the attenuator.
Run the Method
Ensure the Liberty Blue is Ready to Run
1. Verify that the waste container has sufcient capacity.
2. Verify that the ber optic probe is fully inserted into the thermowell.
WARNING
If the probe is not inserted all the way to the bottom of the vessel the Liberty Blue will not accurately
measure the temperature, and signicant overheating of the vessel will occur. This will result in poor
synthesis quality and/or serious damage to the vessel.
3. Verify that the method is correct by looking at the steps outlined in the Liberty Methods widow, and then load the
method from the Methods tab. If there are any errors, make corrections and reload the method.
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