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Quick Setup Guide
3.1. Clear the method by clicking the small X at the top of the resin indicator.
3.2. To correct the method:
3.2.1 Open the Liberty Method Editor.
3.2.2 Open the folder where the method was saved, then click on the method to load it.
3.2.3 Click Save to save the method after editing, then close the Liberty Method Editor.
3.2.4 Load the corrected method as described above.
Run the Method
1. Click the Start button at the top of the Liberty Blue Software main screen.
2. The Loaded Method indicator will turn yellow to indicate the method is running.
3. The Liberty Blue will illuminate all required amino acids positions for the method. Ensure that all reagents
are connected to the correct reagent positions, empty centrifuge tubes are connected to any unused reagent
positions, and that there is sufcient solvent and deprotection solution, then click OK to continue.
4. The Liberty Blue will go through a series of initialization steps and leak checks.
5. During the run, the current step of the method is highlighted in yellow in the Cycle Steps table of the Current Run
tab. A status bar in the lower right of the main screen indicates relative progress of the run.
6. When the run is complete, the Loaded Method indicator will turn green, indicating the method was successfully
completed.
7. When the run is complete, remove the resin from the reaction vessel.
7.1. Remove the reaction vessel from the microwave cavity.
7.2. Unscrew the bottom port of the beige union connector on the vessel drain line. Then, unscrew the
vessel body from the attenuator.
7.3. Using dicholormethane (DCM) or another appropriate solvent, rinse the resin into a clean, empty
centrifuge tube.
NOTE
The Liberty Blue is not equipped to perform peptide cleavage. All cleavage must be carried out at either
room temperature or in the Razor™ Peptide Cleavage system.
7.4. Rinse any resin clinging to the thermowell into the centrifuge tube. Ensure there is no resin on the
top of the reaction vessel attenuator.
7.5. Connect the vessel body to the attenuator, then connect the drain line to the vessel body using the
beige union connector.
7.6. Place the vessel back into the microwave cavity, securing the attenuator.
8. The peptide is now ready to be cleaved and analyzed, and the Liberty Blue is ready for its next run.
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