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Operation of the Liberty Blue
Calibrating Rapid Liberty Blue Microwave Methods
The rapid microwave methods used by the Liberty Blue employ rapid heating rates. For both the deprotection and
coupling methods the set temperature of 90 °C should be reached within 20-30 seconds and maintained for at
least 45 seconds and 100 seconds respectively. The starting temperature for each reaction will be signicantly
warmer than room temperature as residual heat will remain from the previous reaction. The graph below shows
the desired heating prole for Liberty Blue rapid deprotection and coupling microwave methods. The green area
represents the acceptable temperature range.
Cycle Editor
The Cycle Editor allows for the full control and customization of any step of a synthesis. This allows for
optimization of each step of a given peptide synthesis. The Cycle Editor can be accessed from the Edit menu.
The Cycle Editor contains separate cycles for each scale. Within each scale, cycles are sorted by type and then
by resin type, allowing for the development of optimized cycles for each scale. A cycle will only appear in the
Method Editor when creating a method of the same scale.
The Liberty Blue application software comes with optimized default cycles for resin swelling, amino acid addition,
and nal deprotection for each of the available synthetic scales for both standard and high swelling resins. The
default cycle for each scale is listed rst in the Cycle Editor.
Cycle Types
Resin: Resin cycles control the swelling of the resin before synthesis.
Amino Acid: Amino acid cycles control how the deprotection and coupling steps of any one amino acid in the
sequence occur.
Final Deprotection: Final deprotection cycles control the removal of the N-terminal protecting group, and also allow
for N-terminal acetylation of the peptide before cleavage.
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