CEM Liberty Blue User Manual

33
Setting up a Synthesis
Setting up a Synthesis
Loading a Method
Once a method has been created and saved in the Liberty Method Editor, it is available from the Methods tab
on the main Liberty Blue Software screen. To load a method:
1. Open the appropriate folder in the Methods tab.
2. Click on the desired method, and drag the method to the resin position. Alternatively, right-click on the desired
method and select “Queue for run”.
3. The method name will appear in the Loaded Method indicator. The indicator will turn blue, meaning the method
is loaded and ready to run.
Standard Reagents and Concentrations
The Liberty Blue uses stock solutions of all reagents. The table below details the concentrations used on the
Liberty Blue and/or CEM’s preferred reagents.
Reagents 0.005 mmol 0.01 mmol 0.025 mmol 0.05 mmol 0.1 - 0.5 mmol 1.0 - 5.0 mmol
Wash
Solvent*
DMF DMF DMF DMF DMF DMF
Deprotection
Cocktail
4% Piperidine
in DMF
or
2% Piperazine
(w/v) in
EtOH:NMP
(10:90)
4% Piperidine in
DMF
or
2% Piperazine
(w/v) in
EtOH:NMP
(10:90)
20% Piperidine in
DMF
or
10% Piperazine
(w/v) in
EtOH:NMP
(10:90)
20% Piperidine
in DMF
or
10% Piperazine
(w/v) in
EtOH:NMP
(10:90)
20% Piperidine
in DMF
or
10% Piperazine
(w/v) in
EtOH:NMP
(10:90)
20% Piperidine
in DMF
or
10% Piperazine
(w/v) in
EtOH:NMP
(10:90)
Amino Acids 0.04 M in DMF 0.08 M in DMF 0.2 M in DMF 0.2 M in DMF 0.2 M in DMF 0.4 M in DMF
Activator** 0.05 M DIC in DMF 0.10 M DIC in
DMF
0.25 M DIC in DMF 0.50 M DIC in
DMF
1.0 M DIC in
DMF
1.0 M DIC in
DMF
Base 0.05 M Oxyma in
DMF
0.1 M Oxyma in DMF 0.25 M Oxyma in
DMF
0.5 M Oxyma in DMF 1.0 M Oxyma in DMF 1.0 M Oxyma
in DMF
*NMP is suitable as a wash solvent or for dissolution/dilution of reagents, but requires 75°C coupling cycles to reduce the risk of
insertions.
**View the CarboMAX application note, ap0124, at www.cem.com for further details.
NOTE
The Liberty Blue is not equipped to perform peptide cleavages. Cleavages should be carried out at room
temperature or heated in the Razor™.
Recommended Reagents and Strategies for Microwave SPPS on the Liberty Blue
This section is intended to provide general guidance for reagent selection when synthesizing peptides on the
Liberty Blue. For each case CEM’s preferences are ranked in order with #1 being the preferred choice. The
corresponding MW methods recommended are subsequently described in the Microwave Methods Selection.

CEM Liberty Blue Specifications

General

Brand	CEM
Model	Liberty Blue
Category	Laboratory Equipment
Language	English