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Setting up a Synthesis
Preparing Activator Base Solution
1. Measure out the appropriate amount of activator base (as calculated using the Usage and Reagent
Calculators) and transfer to a 250 mL glass bottle.
CAUTION
When using DIEA, NMP must be used as the solvent because DIEA is not miscible in DMF at the standard
concentration of 2 M. The nal concentration of DIEA in the reaction vessel is low enough that miscibility is
not an issue.
2. Add the appropriate volume of solvent to give the correct nal volume.
3. Put a cap on the bottle. Gently swirl the bottle to ensure all of the activator base has gone into solution.
Preparing Amino Acids
1. Weigh out the appropriate amount of each amino acid (as calculated using the Usage and Reagent
Calculators).
2. Transfer each amino acid to a clean 50 mL centrifuge tube.
3. Add the appropriate volume of DMF to each centrifuge tube.
4. Place a cap on each centrifuge tube and shake vigorously to ensure the amino acid has fully gone into solution.
CAUTION
All centrifuge tubes used on the system must be ultra-high performance grade, and must NOT use
CentriStar closures.
Preparing Deprotection Solution
1. Measure out the appropriate amount of deprotection reagent (as calculated using the Usage and Reagent
Calculators).
2. Transfer the deprotection reagent to a 250 mL glass bottle.
3. Add the appropriate volume of DMF to give the correct nal volume as calculated in the Reagent Calculator.
4. Add HOBt or Oxyma if necessary.
5. Place a cap on the bottle. Swirl the bottle gently to ensure the deprotection reagent (and HOBt/Oxyma, if used)
has fully gone into solution.
NOTE
Piperazine may take up to an hour to go into solution. It is helpful to add a magnetic stir bar and stir the
solution to speed dissolution.
Setting up the Liberty Blue
Loading Reagents onto the Liberty Blue
1. Connect amino acid centrifuge tubes to the appropriate positions on the system. Connect empty centrifuge tubes
to all unused positions.
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