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Setting up a Synthesis
Running the Liberty Blue
1. Click the Start button at the top of the Liberty Blue Software main screen.
2. The Loaded Method indicator will turn yellow to indicate the method is running.
3. The Liberty Blue will illuminate all required amino acids positions for the method. Ensure that all reagents are
connected and have sufcient volume, empty centrifuge tubes are connected to any unused reagent positions,
and that there is sufcient solvent and deprotection solution, then click OK to continue.
4. The Liberty Blue will go through a series of initialization steps and leak checks.
5. During the run, the current step of the method is highlighted in yellow in the Cycle Steps table of the Current Run
tab. A status bar in the lower right of the main screen indicates relative progress of the run.
6. When the run is complete, the Loaded Method indicator will turn green, indicating the method was successfully
completed.
Generating A Run History Report
The Run History Report records each command executed by Liberty Blue Software during a run with a date/
time stamp. In addition, the Run History Report records any system errors that occur, allowing for easy
diagnosis and troubleshooting of failed syntheses.
Creating a Run History Report
From the Run History tab:
1. Open the folder for the date on which the method was run.
2. Click the desired run. The run history information will load into the table on the right.
3. Click the Generate Report button.
4. The Report Viewer window will open, and the method report will be rendered as a PDF.
5. Click Save to save the report.
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