17
Operation of the Liberty Blue
NOTE
A Microwave Method created at a specic scale will only be available for use in the Cycle Editor for
cycles created at the same scale.
2. Select a scale and method type (the currently selected parameters will be highlighted). Click out of the lter list
to open the selected cycle type.
3. Click the New Method button.
4. The new Microwave Method will appear in the selected folder. Enter a name for the Microwave Method and
press Enter.
5. Enter the temperature (in °C), Power (in W), Time (in s), and Delta T (in °C) for the rst step in the method.
6. Add the next step to the method by clicking the Add Step button, and then enter the power, temperature, and
time settings for the second step.
7. Repeat step 5 for each step in the method.
8. Enter the amount of time to bubble and the time between each bubbling pulse (the default setting is on for 2
seconds, off for 3 seconds).
9. Save the method by clicking the Save button.
NOTE
To delete a step, click the step to highlight it, then click the Delete Step button. To insert a new step
between existing steps, click a step to highlight it, then click the Insert Step button. The new step will
be inserted above the selected step. To move a step up or down, click the step to highlight it, then click
the Move Up or Move Down buttons.
Editing an Existing Microwave Method
The Microwave Editor allows for settings on existing methods to be modied and saved as needed. The most
common reason to modify an existing method is to increase or decrease the microwave power to optimize peptide
synthesis. To modify an existing method:
1. Click on the lter buttons at the top of the Microwave Editor to open the lter list.
2. Select a scale and method type (the currently selected parameters will be highlighted).Click out of the lter list
to open the selected cycle type.
3. Click on the Microwave Method to be modied.
4. Make any changes as needed.
5. Click Save to save the changes to the method.
Duplicating and Modifying an Existing Microwave Method
Often, it is easier to copy and modify an existing microwave method rather than creating an entirely new method.
Methods can easily be duplicated and modied within the Microwave Editor, and then be moved to different
scale folders as needed. To copy an existing method:
1. Click on the lter buttons at the top of the Microwave Editor to open the lter list.
2. Select a scale and method type (the currently selected parameters will be highlighted).Click out of the lter list
to open the selected cycle type.
3. Click on a Microwave Method to select it and click the Duplicate button.
4. The new Microwave Method will appear named “Copy of (method name)”. Type a new name and press Enter.
5. Make any changes to the method, then click Save to save the modied method.