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Operation of the Liberty Blue
Usage Calculator
The Usage Calculator can be used to determine the amount of each reagent needed for a given method.
Multiple methods can be loaded into the calculator, so that sufcient reagents can be loaded onto the Liberty Blue
for multiple peptides.
To use the Usage Calculator:
1. From the Calculators menu, select Usage Calculator.
2. From the Methods box at the bottom left of the Usage Calculator, right-click the method to be included in the
calculation and select Add to Calculation. The method will then appear in the table at the top left of the Usage
Calculator.
3. The calculator will automatically determine the total usage for each reagent, as well as the mass of each amino
acid required, and estimate the total waste produced.
4. To prepare enough reagents for multiple runs, repeat step 2 for each method to be included in the calculation.
The calculator will automatically update the total usage for each reagent. To remove a selected method from
the calculation, uncheck the box next to the method name. If different amino acid concentrations are required
across the selected runs, a warning will appear to notify that amino acid concentration from the largest scale
will be used in the calculation.
5. To run using a different concentration of amino acid, enter the desired concentration in the box at the top of
the Required Reagent Amounts table. The calculator will automatically update to reect the new concentration.
6. To print a Usage Report, click the View Report button at the bottom of the Usage Calculator screen. This will
generate a report as a PDF which can be printed or saved for future reference.
Reagent Calculator
The Reagent Calculator can be used to determine how to prepare all reagents to be loaded on the system at
the correct concentrations. There are ve tabs: Resin, Deprotection, Activators, Activator Bases, and Amino Acids.
Resin Calculator Tab
1. Enter the scale for the synthesis.
2. Enter the resin substitution value in the Resin Substitution box. This value is provided by the supplier of the
resin, and is usually expressed in mmol/g or meq/g.
3. The mass required will automatically be calculated (in grams).
4. To include this reagent in the Reagent Report, check the Include In Report box.
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