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settling. Note that you can view this graph in “real time” as you are running your sample. It is possible
to capture images of particles that did not trigger the laser or the PMT. This happens because once the
threshold level is met or exceeded, the camera takes a raw image, and collages the raw image based on
your context settings. If there is more than one particle in the raw image, VisualSpreadsheet has no way
to determine which particle produced the trigger event. Therefore the same particle properties (related
to the trigger event, such as CH1 Peak) are assigned to all particles within that raw image. This is the
main reason that your sample should be as close to “One Particle per Used Image” as possible.
The rate at which you set your pump can also influence both the image quality and number of particles
you will collect. If the pump speed is too fast, you may find that the particle that caused the triggering
event is already out of the objective’s field of view before the camera can take an image. This means
that you may have zero particles being captured in your raw images. Slowing down the pump speed can
help alleviate this problem.
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