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Modes of Operation - AutoImage Mode & Trigger Mode
Understanding AutoImage Mode (Including File Reprocessing Mode)
· AutoImage Mode defined
· Particle Concentration Calculation: AutoImage Mode
· Setup & Focus (Camera Image Preview)
· AutoImage Mode (No Save)
· AutoImage Mode
· File Processing Mode (No Save)
· File Processing Mode
Understanding Trigger Mode (Fluorescence & Scatter)
· Trigger Mode defined
· Particle Concentration Calculation: Trigger Mode
· Trigger Mode Setup
· Trigger Mode (No Save)
· Trigger Mode: Fluorescence (Channels 1 & 2) and Laser Light Scatter
· Important Information Regarding Trigger Mode
Understanding AutoImage Mode
AutoImage Mode Definition
AutoImage Mode is an image analysis mode in which the FlowCAM captures images of the moving
fluid at a regular, user-defined interval (Frames Per Second or FPS). This mode is used when
processing samples with high particle concentrations. The total number of particles imaged is a function
of the following:
· concentration and properties of particles in the sample fluid
· camera field of view (FOV) dimensions
· total number of field of view images (taken at a regular, user-defined interval) that were analyzed
The volume analyzed is determined based on flow cell dimensions and the concentration of particles is
reported at the end of the run. In other words, the concentration of particles in the sample fluid is
determined by dividing the total number of particles imaged (counted) by the total volume of sample
fluid imaged. The concentration is automatically calculated using the following information:
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