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Glossary of Particle Properties & Other Terms
The following list of definitions applies to VisualSpreadsheet Version 3.0 particle property fields. Note
that most of these definitions also apply to older versions of VisualSpreadsheet, including 2.4, 2.2, 2.1
and 2.0 software. After each definition, the numbers or values listed in ( ) are the possible range of
values for that particle property.
Area (ABD): Number of pixels in the thresholded (binary) greyscale image converted to a measure of
area by use of the calibration factor. (real > 0)
Aspect Ratio: Width / Length. (real [0, 1]; 1 is the value for a perfect circle; values near zero are for
particles that are long and thin)
Average Blue: Average pixel value of the blue color plane. (real [0, 255]; 255 is intense blue)
Average Green: Average pixel value of the green color plane. (real [0, 255]; 255 is intense green)
Average Red: Average pixel value of the red color plane. (real [0, 255]; 255 is intense red)
Calibration Factor: The conversion factor from pixels to microns (units are microns per pixel). (real,
fixed for each instrument)
Calibration Image: The number of the calibration image that was used in processing the particle.
(integer > 0)
Camera: The camera number.
Capture X: The leftmost X coordinate in pixels of the particle in the original image. (integer in range
of the acceptable region)
Capture Y: The top Y coordinate in pixels of the particle in the original image. (integer in range of the
acceptable region)
Ch1 Area: Area of fluorescence peak of PMT #1 measured over the width (Ch1 Width). (real ≥ 0)
Ch1 Peak: Peak fluorescence value read from PMT #1. (real ≥ 0)
Ch1 Width: Sample width of PMT #1 above the threshold value. (real ≥ 0)
Ch2 Area: Area of fluorescence peak of PMT #2 measured over the width (Ch2 Width). (real ≥ 0)
Ch2 Peak: Peak fluorescence value read from PMT #2. (real ≥ 0)
Ch2 Width: Sample width of PMT #2 above the threshold value. (real ≥ 0)
Ch2/Ch1 Ratio: Ch2 Peak / Ch1 Peak. (real ≥ 0)
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