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Sampling
This section contains three parameters that allow for fine tuning and optimization of the measurements
made on the Trigger Mode channels. These settings have been factory determined and preset to the
optimal default values. Typically, the FlowCAM user should not adjust these settings. However, a brief
description of this feature and each parameter is as follows:
The PMT/Scatter detectors are not monitored simultaneously but are instead sampled by the DSP in a
cyclical fashion taking approximately one µsec per measurement. Switching channels incurs about one
µsec of down time where no measurements can occur. The default configuration is to sample each
channel for 32 measurements before switching to the next channel. When a triggering event is detected
on one of the channels, measurements of the PMTs and scatter detector are recorded for each required
channel using the same sampling cycle as previously described. After the measurements are complete,
the flash LED is illuminated and the camera is triggered.
Sample Width: The total number of measurements to record for each channel for calculating peak and
area values. The peak value and 'area' are calculated by the DSP for each channel and sent to the main
computer. The area value is actually the number of consecutive measurements above the level of 200
(above noise) for the given channel for the trigger event. It is the pulse width of the event on this
channel.
Measurements: The amount of data that is collected on each channel.
Minimum Trigger Pulse Width: The minimum number of consecutive measurements required to
generate a trigger event. Larger values can reduce the probability of random noise generating a trigger
event. A minimum pulse width of two is usually sufficient. A value of one can occasionally result in
false triggering.
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