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Flow Cells & Sample
· Rinse the funnel/sample introduction container between samples to minimize cross
contamination. Pipette tips can also be changed/discarded and replaced with new ones.
· Rinse flow cells after each use.
· Never leave a flow cell with sample or material inside when not in use.
· Clean the exterior of the flow cell before each use with a non-abrasive, lint free cloth.
· Even if a flow cell is unused, the epoxy seals will start to degrade after approximately 12
months. It is recommended to replace a flow cell if:
o the seal between the tubing and glass is broken.
o there is material that has dried on the inside and cannot be removed by cleaning.
o the flow cell is partially to completely clogged.
o the cell is broken.
· An excessive amount of air bubbles in the sample is a sign that the seal on the flow cell may
have been broken.
· One indicator of a clog forming is a reduction in observed particles even when liquid is still
moving through the flow cell.
Field of View (FOV) Flow Cells
· It is recommended to replace a field of view flow cell if:
o the seal between the stainless steel tubing and glass is broken.
o there is material that has dried on the inside and cannot be removed by cleaning.
o the flow cell is irreparably clogged.
o the channel has been broken.
· An excessive amount of air bubbles in the sample is a sign that the seal on the flow cell may
have been broken.
· One indicator of a clog forming is a reduction in observed particles even when liquid is still
moving through the flow cell.
FlowCAM Computer
· The FlowCAM has a specialized PC built into the instrument.
· Never install additional software on the system unless instructed by Fluid Imaging Technologies.
· Do not connect the FlowCAM to the Internet unless instructed to do so by Fluid Imaging
Technologies.
o Internal secure networks are acceptable.
· Periodically transfer data off of the instrument to ensure the hard drive capacity is not exceeded.
· Install all software updates promptly upon receipt.
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